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BI-D1870 acts on the N-terminal AGC kinase domain and completely prevents PMA-induced TSC2 phosphorylation. This chemical, BI-D1870 (CAS 501437-28-1), can vary in color when dissolved in DMSO due to the lot to lot variation in appearance.
Part per million impurities can impart color but will have no bearing on the biological activity of a particular compound.
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BI-D1870 is a cell permeable, ATP-competitive inhibitor of the four isoforms of ribosomal S6 kinase (p90 RSK) (IC50's at 100µM ATP: RSK1 = 31n M, RSK2 = 24n M, RSK3 = 18n M, RSK4 = 15n M).
It does not significantly inhibit 10 other AGC kinases, and over 40 other protein kinases at 100X concentrations.
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